How to Remove Trace Impurities in Chromatography Analytics

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Flow rate: The use of conventional chromatography columns for applications in flow-through (FT) anion exchange chromatography requires high flow rates and production columns need a certain diameter and bed height to achieve a large throughput and therefore have a large bed volume. That is the reason why columns are typically oversized.

Typical process data shows that operation time can be reduced by about 80 per cent and buffer reduction can reach 95 per cent. Protein loading in FT can be two orders of magnitude higher than with columns. Due to the negligible diffusion limitation and the cylindrical construction of the adsorber the high flow rate of the membrane is combined with a large frontal surface resulting in very high throughput and keeping the bed volume small. The recommended flow rate for all the 4 mm Membrane Adsorber is 10-30 bed volumes per minute. Lower flow rates than the recommended flow rates can also be used without any loss of the performance.

Binding capacity: Binding capacity of different molecules was presented in the following table 2. Scaling Studies The capsules - Pico, Nano, Mini , 5”, 10”, 20”, 30” and Mega are designed for simple scale-up. Sartobind Pico (0.08 ml bed volume) is the smallest scalable format in the Sartobind SingleSep family. The Pico, Nano and Mini have the same 4 mm bed height as the manufacturing scale capsules (5”, 10”, 20”, 30” and Mega).

To prove easy scalability, breakthrough performance of different size capsules equipped with Sartobind Q membranes are compared and presented in Figure 3 and 4. The figure 4 shows that the breakthrough performances of all sizes were comparable. In resin-bead chromatography, diffusional limitations cause restriction of flow rate during scale-up to be difficult and expensive.

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