How to Remove Trace Impurities in Chromatography Analytics

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By contrast, scale-up in membrane chromatography is a straightforward, very simple linear procedure. Capacity is directly proportional to membrane surface area (volume) and hence to the size of a membrane unit, where bed height or the number of membrane layers is held constant. For example: After break through experiments with the nano one finds that 200 fold binding capacity is needed. Hence, they will choose the 10 inch capsule, then increase flow rate by a factor of 180 (Table 3).

Conclusion

The breakthrough curves and the dynamic binding capacities show a comparable performance in all Sartobind Q 4 mm capsule sizes and confirms the scalability between different sizes of capsules.

* Authors’ Intro: Sartorius Stedim India

* References

* 1.Zhou J X, Tressel T, Amgen: Membrane chromatography as a robust purification system for large-scale antibody production. Bioprocess International, Sept. 2005, p. 32-37.

* 2.Walter J K, Boehringer-Ingelheim Pharma: Bioseparation and Bioprocess, G. Subramanian (ed.) Wiley VCH, 1998, Vol. II, p. 447–460.

* 3. M. Belanich et al: Reduction of Endotoxin in a Protein Mixture Using Strong Anion Exchange Membrane Adsorption, Pharm. Technology 03, 1996, p. 142–150.

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