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Single Use Bioreactors

Successful Cell–Cultivation in Single Use Bioreactors

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DÜPERTHAL Sicherheitstechnik GmbH & Co. KG
Glatt Ingenieurtechnik GmbH
Angst+Pfister AG
Sartorius AG

Rocking Angle Increses with increasing Oxygen Demand

An initial angle of 6‹ is sufficient. In general, the angle is increased as the oxygen demand increases. When the Cultibag is at 100 per cent of working volume, an angle of 6-10 degrees may be needed. An angle of 6‹ was suitable for cultivation in 2L and 10L Cultibag RM. The angle of rocking can be reduced when excessive foaming is observed.

Single Use Bioreactors Suitable for Sf9 Cell Cultures

Sartorius has demonstrated that the single use bioreactor Biostat Cultibag 20 RM is suitable for the cultivation of sf9 cell lines in serum free chemically defined media. Insect cell lines have higher oxygen demand compared to mammalian cell lines and Cultibag RM is able to support their growth. Single use bioreactors are preferred in various applications in R&D, process development and small scale production. Single use bioreactors remove the requirement for cleaning validation and require minimum maintenance while providing maximum operator ease of use.

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