DIY Assays Kits Develop Label-Free Assays In-House
Pharmaceutical and biotech companies can now develop their own label-free assays using the SoPRano CA1 assay kit from PharmaDiagnostics.
The company claims that this is the first time high-throughput label-free screening has been made available using standard plate readers and simple assay protocols. Users couple their own proteins to PharmaDiagnostics’ SoPRano gold nanoparticles, and carry out the assays using a variant of the well-established surface plasmon resonance (SPR) technique. Applications include fragment screening, focused high-throughput screening (HTS), antibody discovery and hit confirmation. The company also offers SoPRano kits for a range of ADME and physicochemical properties, including plasma protein binding, pKa as well as redox. Previous label-free assays based on SPR have been limited in throughput and have required expensive equipment, says the firm.