Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed. From library generation by random or directed mutagenesis to screening and selection techniques, the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide, that also includes valuable troubleshooting hints on frequently encountered problems. The sequence of chapters mirrors the steps in a standard directed-evolution experiment. In the beginning, various methods for the creation of molecular diversity are considered. As an added bonus, a CD-Rom is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms. The book contains 214 pages and is priced at Euro 129.